P.R.B.P.

PLASMA RICH IN BIOACTIVE PROTEINS
(P.R.B.P.)

Red_White_Blood_cellsOur protocol is a natural and safe way to regeneration of injury by accident or joint wear by the passage of time or for aesthetic purposes. PRBP defenders, promotes as a organically based therapy allows healing by using its own natural stimulation of bioactive proteins.

In recent years, scientific research and technology has given a new perspective on protein. Studies suggest that proteins are an abundance of growth factors and cytokines that may have an effect on inflammation, infection, osteogenesis, regeneration of wounds, muscle injury, and soft tissue healing.

Research now shows that platelets also release many bioactive proteins responsible for the attraction of macrophages, mesenchymal stem cells and osteoblasts that not only promote the elimination of necrotic tissue and degenerate, but also improve tissue regeneration.

The growth of the therapy PRPB has been based mainly on anecdotal reports or cases. Historically, there have been few controlled trials demonstrating the effectiveness of the PRPB.

On the other hand:

  • The lack of consensus in the technic.
  • The number of injections.
  • The spacing of injections.
  • The number of platelets.
  • The platelet concentration over baseline.
  • Leukocytes with or without injection.
  • Injected exogenous platelet activation.
  • And even a definition of suitable candidates for the procedure are lacking and need further definition and evaluation.

It seems intuitive that a count of platelet give more growth factors and better clinical response, however, this has not yet been determined.

PlasmaPRBP contained with white blood cells have different biological activity than the PRBP where they are absent. Some systems platelet blood pool separated into two components: one with the cellular components and the other consists of whey which platelets are suspended. Other separating whole blood into three fractions: red blood cells, serum and buffy coat. The buffy coat containing both platelets and white blood cells (WBCs).

The buffy coat includes neutrophils, monocytes / macrophages, and lymphocytes. Their roles in tissue healing are different. Neutrophils engulf and  contain 40 more hydrolytic enzymes. Their activation leads to phagocytosis of waste and release of oxygen free radicals and proteases. This release of toxic molecules by neutrophils can cause secondary damage to the muscle. It is unknown whether neutrophils have a negative or positive effect on acute or chronic soft tissue injury.

Macrophages are the form of circulating monocytes. Its function is to eliminate waste and its main function is phagocytosis. Also have a role in the balance of pro-inflammatory and anti-inflammatory aspects of healing. Since it is not possible to fractionate the different types of white blood cells out of PRBP, it may be that the absence of macrophages is more damaging to cure any secondary damage caused by neutrophils.