Specialty: Preclinical models, mammalian cell culture, and translational nutraceutical research (Sechium edule, sodium caseinate)
María Guadalupe Ramírez Padilla is a biologist focused on experimental support for hematopoiesis/leukemia, oxidative stress and inflammation, and plant-derived bioactives—particularly Sechium edule hybrids. Her experience includes in-vitro culture of hematologic and solid-tumor cell lines, in-vivo murine handling and dosing (oral, intraperitoneal, subcutaneous), and data collection for translational studies in metabolic and hepatic injury models. She collaborates on IRM scientific projects, connecting laboratory methods with clinically relevant endpoints.
Areas of Expertise
- Mammalian cell culture (e.g., J774, P388, WEHI-3, 4T1, RAW 264.7, L929, A549, 3T3-L1); viability/proliferation assays
- Small-animal handling and dosing in preclinical models (CD-1, BALB/c)
- Experimental design and execution in nutraceutical/functional-food studies (Sechium edule; oxidative & inflammatory markers)
- Basic molecular biology and QA awareness (PCR/primer design; quality-systems training)
Education
- B.Sc. in Biology — FES Zaragoza, Universidad Nacional Autónoma de México (UNAM)
— Professional exam: Hepatoprotective effect of the Sechium HD-Victor hybrid extract (Sept 13, 2021)
Research & Professional Experience
- Hematopoiesis & Leukemia Laboratory (FES Zaragoza, UNAM) — Experimental support in cell culture, animal handling, and student mentoring (2018–present)
- QuantumLab (Puerto Vallarta, Jalisco) — Cell culture biologist (Jan 2023–present)
- Zucarmex (Atencingo, Puebla) — In-vitro sugarcane tissue culture (Dec 2021–Jan 2022)
Research Projects
- PAPIIT IA205222 (UNAM) — Effect of Sechium edule H387-07 on glucose reduction, GLUT4 translocation, and oxidative/inflammatory markers in a diabetic mouse model (Jan 2022–Dec 2023) — Research collaborator
Certifications & Technical Training
- Molecular Biology Diploma — CCBIO (60 h; DC-3 competency certificate), 2024
- Quality in the Clinical Laboratory: Foundations, Implementation & Validation — CCBIO (20 h), 2024
- PCR & Primer Design — CCBIO (15 h), 2023
- Multicolor Flow Cytometry — “Septiembre en Flow” (12 h), 2022
- Beyond the Cell e-Classroom (sorting, single-omics, COVID-19 immune response) — BD/Bociencias, 2020
Conferences & Scientific Forums
- FES Zaragoza Research Congresses — Oral/poster contributions (2019, 2021, 2022)
- Functional Foods & Nutraceuticals Network — Poster: Hepatoprotective activity of the HD-Victor chayote hybrid (Nuevo Vallarta, Nov 2023)
- First Scientific Meeting in Regenerative Medicine (FES Zaragoza) — Talk: Mesenchymal stem cells and cardiovascular diseases (Dec 6, 2024)
Highlighted Scientific Publications
- Hepatoprotective Effect of the Sechium HD-Victor Hybrid Extract in a Carbon-Tetrachloride–Induced Liver-Injury Mouse Model. Biomedicine & Pharmacotherapy 183:117831 (2025). doi:10.1016/j.biopha.2025.117831
— Authors: Santiago-Osorio E., Ramírez-Padilla M.G., Cadena-Íñiguez J., Rosado-Pérez J., et al. - Improved Survival of Leukemic Mice Treated with Sodium Caseinate in Combination with Daunorubicin Without Toxicity. Journal of Oncology 2021:6635650 (2021). doi:10.1155/2021/6635650
— Authors: Aguiñiga-Sánchez I., Meléndez-Ibarra F.M., Ledesma-Martínez E., Weiss-Steider B., Fajardo-Orduña G.R., Rangel-Corona R., Ramírez-Padilla M.G., Santiago-Osorio E., et al.
