Evidence-guided regenerative biotechnology

Personalized protocols designed with explicit safety criteria and objective follow-up. Each preparation is released only after documented quality controls (viability, sterility, endotoxins) and is grounded in three levels of evidence: in vitro studies, preclinical models, and early clinical signals.

Our Scientific Approach

We work with clinical biotechnology and a framework of three levels of evidence: in vitro studies, in vivo validation, and early clinical signals. On that basis, we design personalized protocols under explicit safety, eligibility, and objective follow-up criteria. We do not transfer findings outside their context or communicate benefits without support. Operational details are protected by clinical confidentiality, without affecting the traceability or transparency of results.

Biomaterials and Tissue Microenvironments

We develop and validate bioactive scaffolds and functionalized surfaces (e.g., PCL/PLGA, chitosan, 3D textiles) that replicate features of the extracellular matrix to promote adhesion, protection, and cell signaling. These “microhabitats” aim to guide repair and improve integration with existing tissue, with measurable effects on osteogenic markers and cell–matrix interaction. Any transition to clinical use requires biocompatibility and reproducible performance in preclinical models, with documentation and batch-level traceability.

Immunometabolism and High-Purity Phytoactives

We evaluate standardized bioactive extracts (antioxidant/anti-inflammatory profile) and their impact on oxidative stress, telomeric dynamics, and metabolic markers. The goal is to modulate systemic inflammation and support patient homeostasis within a comprehensive approach. We only consider compounds with analytical purity and traceability; potential uses are integrated when supported by data, communicating scope and limits to avoid extrapolations.

Biosensors, Bioimpedance, and Precision Medicine

We apply electrical bioimpedance, QCM-D, and gene-expression panels to quantify biological processes (inflammation, tissue integrity, microbiota dynamics) and monitor response throughout the therapeutic plan. Together with clinical biomarkers and validated scales, these measures allow us to adjust doses and intervals using objective criteria. We prioritize repeatable measurements, data quality control, and traceability. These are support tools: they do not replace clinical judgment.

Cell Biology and Immune Modulation

Regeneration depends on both cells and the immune milieu. We study how macrophages and other immune cells influence repair: early pro-inflammatory phases can recruit progenitors and activate osteogenic pathways (e.g., BMP-2, RUNX2), while resolution phases promote maturation and remodeling. We explore their modulation via biomolecules and material cues (including nano/hybrid materials), always assessing biocompatibility, safety, and functionality in in vitro and in vivo systems before considering clinical applications.

Immunogenetics and Compatibility

We analyze histocompatibility markers (HLA) and other immunogenetic determinants to support decisions on compatibility, donor selection, and eligibility criteria where applicable. This profile helps anticipate immune interactions and refine strategy in scenarios where the immune system response conditions the outcome. We also consider factors such as immunosenescence and autoimmunity to personalize the protocol. These analyses guide decisions but do not, by themselves, determine the therapeutic indication.

Our Academic Collaborations

We take part in joint projects with universities and research centers that allow us to test methodologies in external settings, ensure that results are reproducible, and maintain an ongoing dialogue with the scientific community. These partnerships provide transparency and independent review, and help ensure that every clinical advance is developed under the same standard of rigor that guides international academic research.

Immunotherapy Regenerative Medicine & UNAM collaboration agreement.

How We Work · Quality and Safety

Priority: safety and objective measurement. Every case begins with a comprehensive clinical assessment and defined eligibility criteria. We only release a preparation if it meets viability, sterility, and endotoxin requirements. Follow-up relies on validated scales and biomarkers; we adjust the strategy based on the data. No outcome guarantees, with batch-level traceability and clinical confidentiality at all times.

  1. Collection / Procurement Documentation and traceability
  2. Fresh Processing Controlled conditions (same day)
  3. Quality Control Viability · Sterility · Endotoxins
  4. Administration IV · IM · Intra-articular

Cell Viability

Proportion of live, functional cells prior to use. Verified with validated quantification methods and exclusion of non-viable cells. Keeping it within range preserves the biological potential and an appropriate risk–benefit profile.

Microbiological Sterility

Confirmation of absence of contamination (bacteria, fungi, or others). Assessed in controlled environments using recognized microbiological tests before batch release. Reduces the risk of infectious events and supports procedural safety.

Endotoxins

Quantification of bacterial endotoxins using validated assays. Meeting the acceptance threshold minimizes undesired systemic reactions. No preparation is administered without first meeting this criterion.

Scientific and Research Team

At Immunotherapy Regenerative Medicine, our team brings together researchers and specialists with expertise in immunology, cell biology, biomaterials, and regenerative medicine. Their combined experience ensures that every development is grounded in published evidence, reproducible methodologies, and documented quality controls.

ALBERTO PARRA BARRERA

Alberto Parra Barrera, Ph.D.

Cell biology and immune modulation applied to regenerative medicine; studies the response of MSCs and macrophages, osteogenic signaling (BMP-2, RUNX2, SPARC), and safety regarding materials such as TiO₂. Evidence: 15 articles (2008–2025) in Cytokine, Neurotoxicology, Scientific Reports, and Journal of Medicinal Food.
EXPERIENCE
Raúl Rosales Ibáñez, Ph.D.

Raúl Rosales Ibáñez, Ph.D.

Biomaterials and tissue engineering; development of bioactive scaffolds and 3D textiles (PCL/PLGA, chitosan) and 3D printing aimed at tissue integration, with in vitro and in vivo results in osteoinduction and angiogenesis. Evidence: 31 articles (2016–2023) in Polymers, J Mater Sci Mater Med, J Biomater Appl, and Biomaterials Science.
EXPERIENCE
Jorge Cadena Iñíguez, Ph.D.

Jorge Cadena Íñiguez, Ph.D.

Phytoactives and immunometabolism; standardization of Sechium extracts and translation into health applications (e.g., glycemic control, hepatoprotection) with an emphasis on preclinical safety and efficacy. Consolidated evidence: >100 articles, 12 books/chapters, 6 patents, and 19 plant variety registrations; recent publications in Biomedicine & Pharmacotherapy, Food Bioscience, and Horticulturae.
EXPERIENCE
Itzen Aguiñiga Sánchez, Ph.D.

Itzen Aguiñiga Sánchez, Ph.D. (SNI Nivel II)

Standardization of bioactive extracts and combinations with antineoplastic agents; research on apoptosis/antiproliferation, oxidative stress, and telomeres in cellular models and cohorts with metabolic syndrome. Evidence: 30 articles (2008–2023) in Nutrients, Molecules, Antioxidants, and Journal of Oncology.
EXPERIENCE
Edelmiro Santiago Osorio, Ph.D.

Edelmiro Santiago Osorio, Ph.D.

Pro-regenerative microenvironments with MSCs of dental origin and antioxidant/anti-inflammatory compounds; applications in periodontics and cardiometabolic aging, including reviews and meta-analyses on glycemic control and oxidative stress. Evidence: 26 articles (2016–2022) in Stem Cells International, International Journal of Molecular Sciences, Antioxidants, and Oxidative Medicine and Cellular Longevity.
EXPERIENCE
Modesto Gómez López Ph.D.

Modesto Gómez López, Ph.D. (SNI Nivel I)

Electrical biosensors and bioimpedance/QCM-D with genetic panels to quantify inflammation, oxidative stress, and clinical response in diabetes/DFU, COVID-19, fertility, and neurodegeneration. Evidence: 24 articles (2017–2025) in Heliyon, International Journal of Molecular Sciences, Journal of Electrical Bioimpedance, and Clinical Oral Investigations.
EXPERIENCE

Scientific Publications and Evidence Base

We clearly present the evidence that underpins our clinical practice. This repository compiles the work of our team and collaborators—peer-reviewed articles, book chapters, and proceedings—organized by lines of research. Each entry specifies the type of study (in vitro, in vivo, clinical), the context, and the real scope of the findings. The presence of a publication does not constitute a therapeutic indication: its interpretation depends on the clinical case and on the quality and relevance of the study.

Biomaterials and Tissue Microenvironments

 

We design and validate bioactive scaffolds and functionalized surfaces (PCL/PLGA, chitosan, composites, and 3D textiles) that act as microhabitats for tissue repair. This line integrates material synthesis, physicochemical characterization, and biological evaluation (adhesion, osteogenesis, angiogenesis, and biocompatibility), with peer-reviewed publications and objective performance metrics.

 

Publications

  1. González-González AM, Cruz R, Rosales-Ibáñez R, Hernández-Sánchez F, Carrillo-Escalante HJ, Rodríguez-Martínez JJ, et al. In vitro and in vivo evaluation of PCL/PLGA scaffold for cartilage injuries. Polymers (Basel). 2023;15:2324. doi:10.3390/polym15102324

  2. Reyna-Urrutia VA, Rosales-Ibáñez R, González-González AM, Estevez M, Rodríguez-Martínez JJ, González-Reyna MA. Biological activity of a chitosan-carboxymethylcellulose-zinc oxide scaffold for bone tissue engineering. J Biomater Appl. 2023;—. doi:10.1177/08853282231168239

  3. Navarro-Cerón A, Barceló-Santana FH, Vera-Graziano R, Rivera-Torres F, Jiménez-Ávila A, Rosales-Ibáñez R, et al. Bovine dentin collagen/PLA scaffolds for teeth tissue regeneration. Iranian Polymer Journal. 2023;—. doi:10.1007/s13726-023-01139-y

  4. Reyna-Urrutia VA, Estevez M, González-González AM, Rosales-Ibáñez R. 3D scaffolds of caprolactone/chitosan/PVA/hydroxyapatite seeded with swine DPSCs. J Mater Sci Mater Med. 2022;33:81. doi:10.1007/s10856-022-06702-2

  5. Cerecedo-Sáenz E, Hernández-Lazcano E, González-Bedolla MJ, Hernández-Ávila J, Rosales-Ibáñez R, Gutiérrez-Amador MP, et al. Potassium jarosite for arsenic removal in contaminated water: preliminary study. Int J Environ Res Public Health. 2022;19(23):15912.

  6. Serralde-Lealba JR, Cerecedo-Sáenz E, Hernández-Ávila J, Arenas-Flores A, Veloz-Rodríguez MA, Gutiérrez-Amador MP, et al. Doped potassium jarosite for bone tissue engineering. Metals (Basel). 2022;12(6):1052. doi:10.3390/met12061052

  7. Rosales-Ibáñez R, Viera-Ruiz AE, Cauich-Rodríguez JV, Carrillo-Escalante HJ, González-González AM, Rodríguez-Martínez JJ, et al. Electrospun/3D-printed PCL bioactive scaffold for bone regeneration. Polymer Bulletin. 2022;—.

  8. Reyna-Urrutia VA, González-González AM, Rosales-Ibáñez R, Estevez M, Rodríguez-Martínez JJ, González-Reyna MA. Scaffolds used in cleft palate regeneration: a review. Polymers (Basel). 2022;14(3):547.

  9. Oliver-Urrutia C, Rosales-Ibáñez R, Flores-Merino MV, et al. Lyophilized polyvinylpyrrolidone hydrogel for culture of human oral mucosa stem cells. Materials (Basel). 2021;14:227.

  10. García Pérez A, González-Aragón Pineda AE, Rosales-Ibáñez R, et al. Association between sociodemographic factors and dental caries in Mexican schoolchildren. Medicine (Baltimore). 2021;100(25):e26435.

  11. Marin-Tapia HA, Romero-Salazar L, Arteaga-Arcos JC, Rosales-Ibáñez R, Mayorga-Rojas M. Micro-mechanical properties of corneal scaffolds from decellularized bi-models. J Mech Behav Biomed Mater. 2021;119:104510. doi:10.1016/j.jmbbm.2021.104510

  12. Rosales-Ibáñez R, Cubo-Mateo N, Rodríguez-Navarrete A, González-González AM, Villamar-Duque TE, Flores-Sánchez LO, et al. Assessment of a PCL-3D printing-dental pulp stem cells triplet for bone engineering: an in vitro study. Polymers (Basel). 2021;13(7):1154. doi:10.3390/polym13071154

  13. Flores-Cedillo ML, Rosales-Ibáñez R, Martín-del-Campo-Fierro M, Reyes-Matute A, Cauich-Rodríguez JV, Oros-Méndez LA, et al. Evaluation of the osteoinductive potential of HDPSCs cultured on MWCNTs/PCL membranes. Polymer Bulletin. 2021;—. doi:10.1007/s00289-021-03721-x

  14. Martín-Pat GE, Rodríguez-Fuentes N, Cervantes-Uc JM, Rosales-Ibáñez R, Carrillo-Escalante HJ, Ku-Gonzalez AF, et al. Effect of iodine-doped polypyrrole plasma treatment on PGS scaffolds. J Biomater Appl. 2020;—. doi:10.1177/0885328220941466

  15. Valadez-González A, Rosales-Ibáñez R, Rodríguez-Navarrete A, Villamar-Duque TE, Cano-Brown J, Carrillo-Escalante HJ, et al. Tailoring surface properties of carbon nanofibers via oxidation and its influence on dental pulp stem cell viability of PCL/CNF composites. Polymer Bulletin. 2020;—. doi:10.1007/s00289-020-03127-1

  16. González-Aragón Pineda AE, García Pérez A, Rosales-Ibáñez R, Stein-Gemora E. Relationship between the normative need for orthodontic treatment and oral health in Mexican adolescents aged 13–15 years old. Int J Environ Res Public Health. 2020;17(21):8107.

  17. Martín-Del-Campo M, Rosales-Ibáñez R, Rojo L. Biomaterials for cleft lip and palate regeneration. Int J Mol Sci. 2019;20(9):—.

  18. Martín-Del-Campo M, Rosales-Ibáñez R, Sampedro JG, Flores-Cedillo ML, Rojo L. Bone regeneration induced by strontium folate loaded biohybrid scaffolds. Molecules. 2019;24(9):—.

  19. Oliver Urrutia C, Rosales-Ibáñez R, Domínguez García MV, Flores-Estrada J, Flores-Merino MV. Synthesis and assessment of poly(acrylic acid)/polyvinylpyrrolidone interpenetrating network as a matrix for oral mucosa cells. J Biomater Appl. 2019;—.

  20. Sánchez-Pech JC, Rosales-Ibanez R, Cauich-Rodriguez JV, Carrillo-Escalante HJ, Rodríguez-Navarrete A, Avila-Ortega A, et al. Design, synthesis, characterization, and cytotoxicity of PCL/PLGA scaffolds through plasma treatment in the presence of pyrrole for urethral tissue engineering. J Biomater Appl. 2019;34(6):840-850. doi:10.1177/0885328219882638

  21. Aguilar-Pérez FJ, Vargas-Coronado RF, Cervantes-Uc JM, Cauich-Rodríguez JV, Rosales-Ibáñez R, Rodríguez-Ortiz JA, et al. Titanium–castor oil-based polyurethane composite foams for bone tissue engineering. J Biomater Sci Polym Ed. 2019;—.

  22. Rodríguez-Méndez I, Fernández-Gutiérrez M, Rodríguez-Navarrete A, Rosales-Ibáñez R, Benito-Garzón L, Vázquez-Lasa B, et al. Bioactive Sr(II)/chitosan/poly(ε-caprolactone) scaffolds for craniofacial tissue regeneration: in vitro and in vivo behavior. Polymers (Basel). 2018;10(3):—.

  23. Guzmán-Uribe D, Alvarado-Estrada KN, Pierdant-Pérez M, Torres-Álvarez B, Sánchez-Aguilar JM, Rosales-Ibáñez R. Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects. J Appl Oral Sci. 2017;25(2):186-195.

  24. Beltrán-Partida E, Valdéz-Salas B, Moreno-Ulloa A, Escamilla A, Curiel MA, Rosales-Ibáñez R, et al. Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes. J Nanobiotechnology. 2017;15:10.

  25. Martin-Del-Campo M, Rosales-Ibañez R, Alvarado K, Sampedro JG, Garcia-Sepulveda CA, Deb S, et al. Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects. Biomater Sci. 2016;4(11):1596-1604.

  26. Ortiz M, Rosales-Ibáñez R, Pozos-Guillén A, de Bien C, Toye D, Flores H, Grandfils C. DPSC colonization of functionalized 3D textiles. J Biomed Mater Res B Appl Biomater. 2016;—.

  27. Santurdes N, González-Gómez A, Martín-del-Campo-Fierro M, Rosales-Ibáñez R, Oros-Ovalle C, Vázquez-Lasa B, San Román J. Development of bioresorbable bilayered systems for application as affordable wound dressings. J Bioact Compat Polym. 2016;31(6):624-647.

  28. Flores-Cedillo ML, Alvarado-Estrada KN, Pozos-Guillén AJ, Murguía-Ibarra JS, Vidal MA, Cervantes-Uc JM, Rosales-Ibáñez R, Cauich-Rodríguez JV. Multiwall carbon nanotubes/polycaprolactone scaffolds seeded with human dental pulp stem cells for bone tissue regeneration. J Mater Sci Mater Med. 2016;27(2):35.

  29. Olvera-Farias RM, Cruz-Perez JR, Salinas-Gutierrez R, Guerrero-Diaz de Leon JA, Kouri-Flores JB, Rosales-Ibáñez R, et al. Unilateral impact of altered loading by changing teeth height on the TMJ fibrocartilage: disc and condyle of Wistar rats. Microscopy Research. 2016;4(2):—.

  30. García Pérez A, Higuera Martínez G, Rosales-Ibáñez R, Hernández González AK, Jiménez Ávila A, Ledesma Soto Y, et al. Aislamiento, cultivo y caracterización de células troncales de médula ósea de fémur de conejos de la raza Nueva Zelanda. Revista indexada nacional. 2018;30:—.

  31. Oliver Urrutia C, Rosales-Ibáñez R, Flores-Merino MV, et al. Synthesis and assessment of poly(acrylic acid)/polyvinylpyrrolidone IPN as a matrix for oral mucosa cells. J Biomater Appl. 2019;—.

Immunometabolism and Bioactives

 

We study standardized bioactive compounds (e.g., Sechium extracts and dairy derivatives such as sodium caseinate) and their relationship with oxidative stress, inflammation, telomere dynamics, and metabolic control. This axis combines in vitro, in vivo, and early clinical evidence to understand how these agents modulate immunometabolic pathways and contribute to more stable biological microenvironments.

 

Publications

  1. Aguiñiga I, Meléndez-Ibarra FM, Ledesma-Martínez E, Weiss-Steider B, Fajardo-Orduna GR, Rangel-Corona R, Santiago-Osorio E. Improved survival of leukemic mice treated with sodium caseinate in combination with daunorubicin without toxicity. J Oncol. 2021;2021:6635650. doi:10.1155/2021/6635650

  2. Rodríguez-Solano L, Aguiñiga I, López-Ortiz M, Tiburcio R, Luviano A, Regla I, Castillo I. Bis(2-methylbenzimidazolyl)amine-derived copper complexes and their antineoplastic activity. Eur J Inorg Chem. 2011;23:3454–3460. doi:10.1002/ejic.201100301

  3. Ramos-Mandujano G, Weiss-Steider B, Melo B, Córdova Y, Ledesma E, Bustos S, Silvestre O, Aguiñiga I, et al. Alpha, beta and kappa caseins inhibit the proliferation of the myeloid cell lines 32D cl3 and WEHI-3 and exhibit different differentiation properties. Immunobiology. 2008;213:133–141. doi:10.1016/j.imbio.2007.07.004

  4. Santiago-Osorio E, Mora L, Bautista M, Montesinos J, Martínez I, Ramos G, Aguiñiga I, et al. Sodium caseinate induces secretion of macrophage colony-stimulating factor from neutrophils. Immunobiology. 2010;215:332–339. doi:10.1016/j.imbio.2009.03.003

  5. Domínguez-Meléndez V, Silvestre-Santana O, Moreno-Fierros L, Aguiñiga I, et al. Sodium caseinate induces mouse granulopoiesis. Inflamm Res. 2012;61(4):367–373. doi:10.1007/s00011-011-0421-7

  6. Ledesma-Martínez E, Pérez-Cordero C, Córdova-Galaviz Y, Sánchez-Téllez G, Huerta-Yépez S, Aguiñiga I, et al. Casein induces apoptosis of WEHI-3 leukaemic cells and increased survival in a leukaemic mouse model. Oncol Lett. 2012;4(3):461–466. doi:10.3892/ol.2012.758

  7. Luviano A, Aguiñiga I, Demare P, Tiburcio R, Ledesma-Martínez E, Santiago-Osorio E, Regla I. Antineoplastic activity of rinvanil and phenylacetylrinvanil in leukaemia cell lines. Oncol Lett. 2014;7(5):1651–1656. doi:10.3892/ol.2014.1958

  8. Córdova-Galaviz Y, Ledesma-Martínez E, Aguiñiga I, Soldevila-Melgarejo G, Soto-García I, Weiss-Steider B, Santiago-Osorio E. Sodium caseinate induces increased survival in leukaemic mouse J774 model. In Vivo. 2014;28(5):819–825.

  9. Aguiñiga I, Soto-Hernández M, Cadena-Íñiguez J, Ruíz-Posadas L, Cadena-Zamudio J, González-Ugarte A, Weiss-Steider B, Santiago-Osorio E. Fruit extract from a Sechium edule hybrid induces apoptosis in leukaemic cell lines but not in normal cells. Nutr Cancer. 2015;67(2):250–257. doi:10.1080/01635581.2015.989370

  10. Castillo I, Suwalsky M, Gallardo M, Troncoso V, Sánchez-Eguía B, Santiago-Osorio E, Aguiñiga-Sánchez I, González-Ugarte A. Structural and functional effects of benzimidazole/thioether–copper complexes with antitumor activity on cell membranes and molecular models. J Inorg Biochem. 2016;156:98–104. doi:10.1016/j.jinorgbio.2015.12.022

  11. Domínguez-Meléndez V, Aguiñiga I, Moreno-Fierros L, Torres B, Osorio E. El caseinato de sodio incrementa el número de linfocitos B en ratón. Rev Biomed. 2017;37(4):571–576. doi:10.7705/biomedica.v37i4.3604

  12. Aguiñiga I, Cadena-Íñiguez J, Santiago-Osorio E, Gómez-García G, Mendoza-Núñez VM, Rosado-Pérez J, Ruiz-Ramos M, Ledesma-Martínez E, Delgado-Bordonave AJ, Cisneros-Solano VM, Soto-Hernández RM. Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum. Pharm Biol. 2017;55(1):1638–1645. doi:10.1080/13880209.2017.1316746

  13. Salazar-Aguilar S, Ruiz-Posadas L, Cadena-Íñiguez J, Soto-Hernández M, Santiago-Osorio E, Aguiñiga I, et al. Sechium edule (Jacq.) Swartz, a new cultivar with antiproliferative potential in a human cervical cancer HeLa cell line. Nutrients. 2017;9(8):798. doi:10.3390/nu9080798

  14. Suwalsky M, Castillo I, Sánchez-Eguía B, Gallardo M, Dukes N, Santiago-Osorio E, Aguiñiga I, Rivera-Martínez A. In vitro effects of benzimidazole/thioether-copper complexes with antitumor activity on human erythrocytes. J Inorg Biochem. 2018;178:87–93. doi:10.1016/j.jinorgbio.2017.10.009

  15. Rosado-Pérez J, Aguiñiga I, Arista-Ugalde TL, Santiago-Osorio E, Mendoza-Núñez VM. The biological significance of oxidative stress, effects of fruits as natural edible antioxidants. Curr Pharm Des. 2018;24(40):4807–4824. doi:10.2174/1381612824666190114164758

  16. Weiss-Steider B, Córdova Y, Aguiñiga I, Ledesma-Martínez E, Domínguez-Meléndez V, Santiago-Osorio E. El caseinato de sodio y la α-caseína inhiben la proliferación de la línea celular mieloide de ratón 32D cl3 vía TNF-α. Biomedica. 2019;39(3). doi:10.7705/biomedica.v39i3.4094

  17. Rosado-Pérez J, Aguiñiga I, Santiago-Osorio E, Mendoza-Núñez VM. Effect of Sechium edule var. nigrum spinosum (Chayote) on oxidative stress and pro-inflammatory markers in older adults with metabolic syndrome: An exploratory study. Antioxidants. 2019;8(5):146. doi:10.3390/antiox8050146

  18. Ledesma-Martínez E, Aguiñiga I, Weiss-Steider B, Rivera-Martínez AR, Santiago-Osorio E. Casein and peptides derived from casein as antileukaemic agents. J Oncol. 2019;2019:8150967. doi:10.1155/2019/8150967

  19. Gavia-García G, Rosado-Pérez J, Aguiñiga I, Santiago-Osorio E, Mendoza-Núñez VM. Effect of Sechium edule var. nigrum spinosum on telomerase levels and antioxidant capacity in older adults with metabolic syndrome. Antioxidants. 2020;9(7):634. doi:10.3390/antiox9070634

  20. Aguiñiga I, Soto-Hernández M, Cadena-Íñiguez J, Suwalsky M, Colina JR, Castillo I, Santiago-Osorio E. Phytochemical analysis and antioxidant and anti-inflammatory capacity of the extracts of fruits of the Sechium hybrid. Molecules. 2020;25(20):4637. doi:10.3390/molecules25204637

  21. Gavia-García G, Rosado-Pérez J, Arista-Ugalde TL, Aguiñiga I, Santiago-Osorio E, Mendoza-Núñez VM. Telomere length and oxidative stress and its relation with metabolic syndrome components in the aging. Biology. 2021;10(4):253. doi:10.3390/biology10040253

  22. Fajardo-Orduna GR, Ledesma-Martínez E, Aguiñiga I, Mora-García ML, Weiss-Steider B, Santiago-Osorio E. Inhibitors of chemoresistance pathways in combination with Ara-C to overcome multidrug resistance in AML: A mini review. Int J Mol Sci. 2021;22:4955. doi:10.3390/ijms22094955

  23. Salazar-Aguilar S, Ruiz-Posadas L, Cadena-Íñiguez J, Soto-Hernández M, Santiago-Osorio E, Aguiñiga I, et al. Microencapsulation of an extract of Sechium edule (Jacq.) Sw. with antineoplastic activity. Appl Sci. 2021;12:—.

  24. Cadena-Íñiguez J, Aguiñiga I, Uriostegui-Arias MT, Santiago-Osorio E, Ruiz-Posadas L, Soto-Hernández M. Antiproliferative effect of Sechium edule cv. Madre Negra extracts on breast cancer in vitro. Separations. 2022;9(9):230. doi:10.3390/separations9090230

  25. Delgado-Tiburcio EE, Cadena-Íñiguez J, Santiago-Osorio E, Ruiz-Posadas L, Castillo-Juárez I, Aguiñiga I, Soto-Hernández M. Pharmacokinetics and biological activity of cucurbitacins. Pharmaceuticals. 2022;15(11):1325. doi:10.3390/ph15111325

  26. Arista-Ugalde TL, Santiago-Osorio E, Monroy-García A, Rosado-Pérez J, Aguiñiga I, Cadena-Íñiguez J, Mendoza-Núñez VM. Antioxidant and anti-inflammatory effect of the consumption of powdered concentrate of Sechium edule var. nigrum spinosum in Mexican older adults with metabolic syndrome. Antioxidants. 2022;11(6):1076. doi:10.3390/antiox11061076

  27. Aguiñiga I, Ledesma-Martínez E, Lara-Castañeda JL, Meléndez-Ibarra FM, Weiss-Steider B, Soto-Cruz I, Santiago-Osorio E. Sodium caseinate in combination with daunorubicin or cytarabine improves survival of mice with long-established leukemia. Cancer Diagn Progn. 2022;2(4):496–502. doi:10.21873/cdp.10133

  28. Rivera-Martínez AR, Aguiñiga I, Cadena-Íñiguez J, Soto-Cruz I, Monroy-García A, Gómez-García G, Santiago-Osorio E. Fruit extract of Sechium chinantlense induces apoptosis in the human cervical cancer HeLa cell line. Nutrients. 2023;15(3):667. doi:10.3390/nu15030667

  29. Hernández-Álvarez D, Rosado-Pérez J, Gavia-García G, Arista-Ugalde TL, Aguiñiga I, Santiago-Osorio E, Mendoza-Núñez VM. Aging, physical exercise, telomeres, and sarcopenia: A narrative review. Biomedicines. 2023;11(2):598. doi:10.3390/biomedicines11020598

  30. Gavia-García G, Rosado-Pérez J, Arista-Ugalde TL, Aguiñiga I, Santiago-Osorio E, Mendoza-Núñez VM. The consumption of Sechium edule has antioxidant effect and prevents telomere attrition in older adults with metabolic syndrome. Redox Rep. 2023;28(1):2207323. doi:10.1080/13510002.2023.2207323

  31. Ledesma-Martínez E, Mendoza-Núñez VM, Santiago-Osorio E. Mesenchymal stem cells derived from dental pulp: a review. Stem Cells Int. 2016;2016:4709572. doi:10.1155/2016/4709572

  32. Fajardo-Orduna G, Mayani H, Castro-Manrreza M, Flores-Figueroa E, Flores-Guzmán P, Arriaga-Pizano L, Santiago-Osorio E, Legorreta-Haquet M. Bone marrow mesenchymal stromal cells from clinical scale culture: in vitro evaluation of their differentiation, hematopoietic support, and immunosuppressive capacities. Stem Cells Dev. 2016;25(17):1299–1310. doi:10.1089/scd.2016.0071

  33. Hernández-Monjaraz B, Santiago-Osorio E, Monroy-García A, Ledesma-Martínez E, Mendoza-Núñez VM. Mesenchymal stem cells of dental origin for inducing tissue regeneration in periodontitis: A mini-review. Int J Mol Sci. 2018;19(4):944. doi:10.3390/ijms19040944

  34. Mendoza-Núñez VM, Arista-Ugalde TL, Rosado-Pérez J, Ruiz-Ramos M, Santiago-Osorio E. Hypoglycemic and antioxidant effect of Tai chi exercise training in older adults with metabolic syndrome. Clin Interv Aging. 2018;13:523. doi:10.2147/CIA.S157584

  35. Hernández-Monjaraz B, Santiago-Osorio E, Ledesma-Martínez E, Alcauter-Zavala A, Mendoza-Núñez VM. Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: a case report. J Int Med Res. 2018;46(7):2983–2993. doi:10.1177/0300060518773244

  36. Ledesma-Martínez E, Mendoza-Núñez VM, Santiago-Osorio E. Mesenchymal stem cells for periodontal tissue regeneration in elderly patients. J Gerontol A Biol Sci Med Sci. 2019;74(9):1351–1358. doi:10.1093/gerona/gly227

  37. Mendoza-Núñez VM, García-Martínez BI, Rosado-Pérez J, Santiago-Osorio E, Pedraza-Chaverri J, Hernández-Abad VJ. The effect of 600 mg alpha-lipoic acid supplementation on oxidative stress, inflammation, and RAGE in older adults with type 2 diabetes mellitus. Oxid Med Cell Longev. 2019;2019:3276958. doi:10.1155/2019/3276958

  38. Hernández-Monjaraz B, Santiago-Osorio E, Ledesma-Martínez E, Aguiñiga-Sánchez I, Sosa-Hernández NA, Mendoza-Núñez VM. Dental pulp mesenchymal stem cells as a treatment for periodontal disease in older adults. Stem Cells Int. 2020;2020:8890873. doi:10.1155/2020/8890873

  39. García-Martínez BI, Ruiz-Ramos M, Pedraza-Chaverri J, Santiago-Osorio E, Mendoza-Núñez VM. Hypoglycemic effect of resveratrol: a systematic review and meta-analysis. Antioxidants. 2021;10(1):69. doi:10.3390/antiox10010069

  40. García-Martínez BI, Ruiz-Ramos M, Pedraza-Chaverri J, Santiago-Osorio E, Mendoza-Núñez VM. Influence of age and dose on the effect of resveratrol for glycemic control in type 2 diabetes mellitus: Systematic review and meta-analysis. Molecules. 2022;27:5232. doi:10.3390/molecules27165232

  41. Osorio ES, Ramírez-Padilla MG, Medina VM, Cadena JI, Hernández MH, Rosado JP, Romero LE, Weiss SB, Gavia GG, Arista TL, Cruz VM, Aguiñiga-Sánchez I. Hepatoprotective effect of the Sechium HD-Victor hybrid extract in a model of liver damage induced by carbon tetrachloride in mice. Biomed Pharmacother. 2025;183:117831. doi:10.1016/j.biopha.2025.117831

Biosensors, Bioimpedance, and Precision Medicine

 

We apply electrical and molecular measurement technologies (bioimpedance, QCM-D, genetic panels, and gene expression) to quantify biological processes such as inflammation and oxidative stress, and to monitor responses to interventions. This axis connects instrumentation, signal analysis, and early clinical validation in areas including diabetes, infections, infertility, and neurodegeneration.

 

Publications

  1. Arias-González L, Cruz-Ramos JA, Pérez-Vielma NM, Munguía-Cervantes JE, Hernández-Sánchez E, Rojas López M, Gómez-López M. Correlation of EBIS vs. concentration and purity of total DNA in breast cancer samples. J Phys Conf Ser. 2025;3014:012011.

  2. Cabrera Catalán B, Cleva Villanueva G, Pérez-Vielma NM, Lara Padilla E, Romero López E, Herrera López ME, Camberos Schiaffini DD, Gómez-López M. Use of bioimpedance as an early diagnosis tool in patients with diabetes. J Phys Conf Ser. 2025;3014:012003. doi:10.1088/1742-6596/3014/1/012003

  3. Valencia-Gutiérrez MM, Gómez-López M, Pérez-Vielma NM, Lázaro-Aguilar P, Aguilera-Sosa VR. Características clínicas y expresión génica de citocinas en síndrome post-COVID-19. Rev Med Inst Mex Seguro Soc. 2025;63(1):e6501.

  4. Pérez-Vielma NM, Gómez-López M, Martínez-Godínez MA, Luna-Torres AL, Domínguez-López A, Miliar-García Á. Candida variety in the oral cavity of Mexican subjects with type 2 diabetes mellitus and TLR2 gene expression. Clin Pract. 2024;14:417-425. doi:10.3390/clinpract14020031

  5. Pérez-Vielma NM, Valencia-Gutiérrez MM, Sánchez-Camacho JV, González-Hernández JE, Miliar-García Á, Ochoa C, Labovitz J, Gómez-López M. Hyperbaric oxygen therapy and oxidative stress/inflammation in diabetic foot ulcers: a preliminary study. Heliyon. 2024;10:e40586. doi:10.1016/j.heliyon.2024.e40586

  6. Sánchez-Camacho JV, Gómez-Chavarín M, Galindo-Solano N, Padilla-Cortés P, Maldonado-García JL, Pérez-Sánchez G, Pavón L, Ramírez-Santos J, Roldán R, Gómez-López M, Gutiérrez-Ospina G. Non-categorical analyses identify rotenone-induced ‘Parkinsonian’ rats benefiting from nano-emulsified punicic acid. Int J Mol Sci. 2024;25:12635. doi:10.3390/ijms252312635

  7. González-Díaz CA, Suárez-Souto MA, Pérez-Soto E, Gómez-López M, et al. OGG1 Ser326Cys polymorphism in infertile men. Biomedicines. 2024;12:2286. doi:10.3390/biomedicines12102286

  8. Morán-Guel E, Gómez-López M, Delgado-Aguirre HA. Factores de riesgo para infección adquirida en el hospital en COVID-19. Med Crit. 2024;38(3):162-168. doi:10.35366/117778

  9. Pérez-Vielma NM, Gómez-López M, Maldonado-Vázquez J, Correa-Basurto J, Martínez-Godínez MA, Miliar-García Á. Interaction between valpropro peptide and sod/cat minimal promoter region by QCM-D and docking. Anal Methods. 2023;—.

  10. Padilla-García KL, Gómez-López M, Sánchez-Camacho JV, Andrade-Torres CM, Pérez-Vielma NM, González-Díaz CA. Bioimpedance spectra in final RT-PCR products: sensitivity threshold analysis. In: CNIB Proc. 2023:665-673.

  11. Ángeles-Estrada L, Pérez-Soto E, Pérez-Vielma NM, Gómez-López M, Sánchez-Monroy V. Oxidative stress and genotoxicity in oral epithelial cells from orthodontic patients. Clin Oral Investig. 2023;—.

  12. Carrizalez-Luna JE, Reséndiz-Albor AA, Arciniega-Martínez IM, Gómez-López M, Campos-Rodríguez R, Pacheco-Yépez JC, Drago-Serrano ME. Outcomes of nicotinic modulation on intestinal IgA response. Biomed Rep. 2023;13:—.

  13. Rojas-Ortega DA, Rojas-Hernández S, Sánchez-Mendoza ME, Gómez-López M, Sánchez-Camacho JV, Rosales-Cruz E, Carrasco-Yépez MM. Role of FcγRIII in BALB/c mice in primary amebic meningoencephalitis. Parasitol Res. 2023;—. doi:10.1007/s00436-023-07810-w

  14. Ames-Lastra G, Castillo-Salazar IA, Hernández-Nava A, Sánchez V, Gómez-López M, Pérez-Vielma N, González-Díaz CA. DNA biosensor by relative bioimpedance and genetic amplification: preliminary results. J Phys Conf Ser. 2021;—.

  15. Ames-Lastra G, Sánchez V, Sacristán-Rock E, Gómez-López M, Pérez-Vielma N, Castillo-Salazar IA, Hernández-Nava A, González-Díaz CA. Bioimpedance spectroscopy to detect label-free PCR products. J Phys Conf Ser. 2021;—.

  16. Hernández A, Ames G, Sánchez V, Gómez-López M, Sánchez J, Pérez N, Castillo IA, González CA. Correlation between DNA concentration and electrical bioimpedance. J Phys Conf Ser. 2021;—.

  17. Marín-Soto MD, Miliar-García Á, Gómez-López M, González-Mundo I, Aguilera-Sosa VR. Association between food craving and taste genes in people with obesity. Invest Clin. 2021;62(2):119-131. doi:10.22209/IC.v62n2a03

  18. Sandoval-Valerio AK, Aguilera-Sosa VR, Miliar-García Á, Gómez-López M, García-García C, Leija-Alva G, Sánchez-Camacho JV, Pérez-Vielma NM. Modulation of catastrophic thinking, pain and inflammation in fibromyalgia. Invest Clin. 2021;62(2):140-158. doi:10.22209/IC.v62n2a05

  19. González-Mundo I, Pérez-Vielma NM, Gómez-López M, Fleury A, Correa-Basurto J, Rosales-Hernández MC, Sixto-López Y, Martínez-Godínez MA, Domínguez-López A, Miliar-García Á. DNA methylation of REST and antioxidant enzyme expression in Alzheimer’s disease. Exp Gerontol. 2020;136:110951. doi:10.1016/j.exger.2020.110951

  20. Gómez-López M, Miliar-García Á, Pérez-Vielma NM, Lara-Padilla E, González-Díaz CA. Biosensor of inflammation biomarkers based on electrical bioimpedance. J Electr Bioimp. 2020;11:31-37. doi:10.2478/joeb-2020-0006

  21. Gómez-López M, Miliar-García Á, Pérez-Vielma NM, Ames-Lastra G, Lara-Padilla E, Chávez KV, García AR, Delgado J, Cortés E, González-Díaz CA. NLRP3 bioimpedance biosensor. IFMBE Proc. 2019;75:1488-1493. doi:10.1007/978-3-030-30648-9_191

  22. Sandoval-Valerio AK, Pérez-Vielma NM, Miliar-García Á, Gómez-López M, García-García C, Aguilera-Sosa VR. Negative thoughts and stress associated with 5HT1A receptor in women with fibromyalgia. Acta Invest Psicológica. 2020;10(3):—. doi:10.22201/fpsi.20074719e.2020.3.3619

  23. Gómez-López M, Miliar-García Á, Pérez-Vielma NM, Ames-Lastra G, Lara-Padilla E, Chávez KV, García AR, Delgado J, Cortés E, González-Díaz CA. NLRP3 bioimpedance biosensor (conference version). IFMBE Proc. 2019;75:—. doi:10.1007/978-3-030-30648-9_19

  24. Gómez-López M, et al. Biosensor de ADN complementario basado en bioimpedancia eléctrica. COMIB/CNIB. 2017;—. doi:10.24254/CNIB.17.93

  25. Gómez-Conde E, Díaz-Orea MA, Pimentel-Morales A, Cárdenas-Perea ME, Galicia-Zamalloa AL, Guerrero-González T, González-Barrios JA, Gómez-López M, Lara-Padilla E. MAP4/MAP6 expression in breast cancer cell lines. Int J Clin Exp Pathol. 2017;10(6):7017-7022.

Cell Biology and Immune Modulation

 

We investigate how different stimuli—hormonal, nutraceutical, and biomedical materials—regulate key cellular pathways (macrophages, oxidative stress, osteogenesis) and cell–material interactions. We combine in vitro assays, in vivo studies, and expression analyses to identify markers of safety, viability, and differentiation in human cells (e.g., MSCs), as well as immunomodulatory effects with translational potential.

 

Publications

  1. Parra-Barrera A, et al. Creación exitosa de neoesófago mediante ingeniería de tejidos en un modelo animal. Rev Mex Cir Pediatr. 2008;15(2).

  2. Parra-Barrera A, et al. Células troncales mesenquimales autólogas e injerto cutáneo autólogo para úlcera crónica secundaria a diabetes mellitus tipo 2. Cir Cir. 2015;83(6).

  3. Parra-Barrera A, et al. Inhibition of the Na+/H+ antiporter induces cell death in TF-1 erythroleukemia cells stimulated by stem cell factor. Cytokine. 2015;75:142–150.

  4. Parra-Barrera A, et al. Internalization of titanium dioxide nanoparticles by glial cells is given at short times and is mainly mediated by actin reorganization-dependent endocytosis. Neurotoxicology. 2015;51:27–37.

  5. Parra-Barrera A, et al. Effect of dehydroepiandrosterone on expression of BMP2, SPARC and RUNX2 in human bone marrow mesenchymal stem cells. Rev Mex Ing Quim. 2016;15(1).

  6. Parra-Barrera A, et al. Comparación entre células troncales mesenquimales obtenidas de médula ósea, tejido adiposo y gelatina de Wharton con base en los criterios de la ISCT. Rev Mex Ing Bioméd. 2017;38(1):280–287.

  7. Parra-Barrera A, et al. Efecto del medio condicionado de H9C2 tratada con dehidroepiandrosterona y expuesta a daño sobre la motilidad de MSC de médula ósea. Rev Mex Ing Bioméd. 2017;38(1):256–263.

  8. Parra-Barrera A, et al. Efecto de la dehidroepiandrosterona y el acetónido de triamcinolona sobre la línea celular 3T3-L1. Rev Mex Ing Bioméd. 2017;38(1):288–296.

  9. Parra-Barrera A, et al. How the infectious agents are involved in childhood leukemia. Arch Med Res. 2017;48:305–315.

  10. Palma-Lara I, Calzada-Mendoza CC, Mera-Jiménez E, Romero López E, Amaya-Espinoza JL, Parra-Barrera A, Gutiérrez-Iglesias G. Medicina regenerativa para úlceras crónicas: aplicación cutánea de MSC de gelatina de Wharton. Rev Mex Ing Bioméd. 2019;40(2):1–11.

  11. Aguayo-Cerón KA, Morales-González JA, Gutiérrez-Iglesias G, Parra-Barrera A, Jiménez-Zamarripa CA, Ocharán-Hernández ME, Calzada-Mendoza CC. Efecto regulador de tibolona sobre la actividad antiinflamatoria del macrófago. Rev Mex Ing Bioméd. 2019;40(3):1–10. doi:10.17488/RMIB.40.3.1

  12. Peralta-Vega AB, Parra-Barrera A, Ramos-Godínez MP, López-Marure R, Arellano-Galindo J, Gutiérrez-Iglesias G. Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells. Mol Cell Toxicol. 2020;16:321–330. doi:10.1007/s13273-020-00084-8

  13. Flores-Mejía R, Fragoso-Vázquez MJ, Pérez-Blas LG, Parra-Barrera A, Hernández-Castro SS, Estrada-Pérez AR, Rodríguez J, Lara-Padilla E, Ortiz-Morales A, Correa-Basurto J. Chemical characterization (LC–MS–ESI), cytotoxic activity and intracellular localization of PAMAM G4 in leukemia cells. Sci Rep. 2021;11:8210. doi:10.1038/s41598-021-87560-w

  14. Aguayo-Cerón KA, Gutiérrez-Iglesias G, Parra-Barrera A, Ocharan-Hernández ME, Romero-Nava R, Jiménez-Zamarripa CA, Calzada-Mendoza CC. Antisclerothic effect of tibolone by reducing pro-inflammatory cytokines expression, ROS production and LDL-ox uptake in THP-1 macrophages. Steroids. 2021;167:108779.

  15. Palma-Lara I, Calzada-Mendoza CC, Mera-Jiménez E, Romero López E, Amaya-Espinoza JL, Parra-Barrera A, Gutiérrez-Iglesias G. (−)-Epicatechin promotes bone regeneration by inducing BMP2, SPARC and RUNX2 expression in mesenchymal stem cells in vitro. J Med Food. 2025;[Epub ahead of print]. doi:10.1089/jmf.2024.0256

Immunogenetics and Compatibility

 

We analyze histocompatibility markers (HLA) and other immunogenetic determinants to support safe decisions on compatibility, donor selection, and eligibility criteria. This line links HLA typing, population registries, and standardized technical documentation with clinical applications where the immune response is decisive.

Publications

Artículos en revistas

  1. Rangel R, et al. Macrolide-Clarithromycin Task-Force for the Treatment and Prophylaxis of COVID-19 as a Single Agent. Pharmacology & Pharmacy. 2020;11(6):131–142. doi:10.4236/pp.2020.116012

Otras publicaciones académicas (proceedings, manual técnico)
2. Rangel R, Alaez C, Berumen J, Gorodezky C. Involvement of HLA class I and class II loci in the expression of cervical cancer in Mexican-mestizo women in the context of human papilloma virus. Hum Immunol. 2001;62(Suppl 1):S83.
3. Flores H, Alaez C, Hernández M, Arroyo M, Rangel R, et al. Data analysis of the Mexican marrow donor registry (DONORMO). Hum Immunol. 2001;62(Suppl 1):S169.
4. Flores H, Alaez C, Rangel R, et al. HLA class I antigens and DRB1, DQA1 and DQB1 alleles in patients with chronic myeloid leukemia (CML) belonging to 150 families. Clinical Histocompatibility Workshop Visuals. 2002:85–86.
5. Organización Panamericana de la Salud; INDRE. Manual de procedimientos serológicos y celulares de histocompatibilidad. 1999. (Manual técnico institucional).

The publications listed here document methods and results; not all of them evaluate the same interventions we offer in the clinic. Findings should not be extrapolated beyond their context. For the criteria and controls applicable to our processes, see the section How We Work · Quality and Safety.

Our Immunotherapy NK Division

All information related to cancer care is centralized in Immunotherapy NK, where the entire care pathway is documented with case-level traceability: initial evaluation, inclusion/exclusion criteria, therapeutic scheme, and monitoring with biomarkers and validated scales. Each protocol details its scientific rationale, scope and limitations, as well as the safety requirements that must be met prior to implementation.

How we treat cancer
Cancer clinic in Mexico – certified integrative and regenerative care